Gastrulação

Interesse da Investigação

Study of the genes and processes involved in the formation of the head field and the associated process of anterior neural induction of the vertebrate embryo. For this purpose, we use the mouse, Xenopus and chick embryos as model systems using the most up to date technologies in molecular embryology. Those include generation and analysis of KO and transgenic mouse lines, microinjection into Xenopus embryos and chick electroporation.

Publicações

Kimura-Yoshida, C., Nakano, H., Okamura, D., Nakao, K., Yonemura, S., Belo, J.A., Aizawa, S., Matsui, Y. and Matsuo, I. (2005). Canonical Wnt signaling and its antagonist regulate anterior-posterior axis polarization by guiding cell migration in mouse visceral endoderm. Dev. Cell 9 :639-650

Echevarria, D., Martínez, S., Marques, S., Lucas-Teixeira, V. and Belo, J.A. (2005). Mkp3 is a negative feedback modulator of Fgf8 signaling in the mammalian isthmic organizer. Dev. Biology 277(1) :114-128

Mesnard, D.*, Filipe, M.*, Belo, J.A. and Zernicka-Goetz, M. (* equal authors) (2004). Emergence of the anterior-posterior axis after implantation relates to the re-orienting symmetry of the mouse embryo rather than the uterine axis. Current Biology 14 :184-196

Marques, S., Borges, A.C., Silva, A.C., Freitas, S., Cordenonsi, M., and Belo, J.A. (2004). The activity of the Nodal antagonist Cerl-2 in the mouse node is required for correct L/R body axis. Genes & Development 18 :2342-2347

Silva, A., Filipe, M., Kuerner, K., Steinbeisser, H. and Belo, J.A. (2003). Endogenous Cerberus activity is required for anterior head induction in Xenopus. Development 130 :4943-4953

Borges, A.C., Marques, S. and Belo, J.A (2002). Goosecoid and cerberus-like do not interact during mouse embryogenesis Int. J. Dev. Biol 46 :259-262

Borges, A.C., Marques, S. and Belo, J.A. (2001). The BMP antagonists cerberus-like and noggin do not interact during mouse forebrain development. Int. J. Dev. Biol. 45 :441-443

Bachiller, D., Klingensmith, J., Kemp, C., Belo, J.A., Anderson, R.M., May, S.R., McMahon, J.A., McMahon, A.P., Harland, R.M., Rossant, J. and De Robertis, E.M. (2000). The organizer secreted factors chordin and noggin are required for forebrain development in the mouse. Nature 403 :658-661

Belo, J.A., Bachiller ,D., Agius, E., Kemp, C., Borges, A.C., Marques, S., Piccolo, S. and De Robertis, E.M. (2000). Cerberus-like is a BMP4 and Nodal antagonist not essential for mouse development. Genesis 26 :259-264

Zhu, L., Belo, J.A., De Robertis, E.M. and Stern, C.D. (1999). Neural inducing strength of mouse organizer controlled by goosecoid dosage. Dev. Biol. 216 :276-281

Belo, J.A., Leyns, L., Yamada, G. and De Robertis, E.M. (1998). The prechordal midline of the chondrocranium is defective in Goosecoid-1 mouse mutants. Mech. Dev. (issue cover) 72 :15-25

Belo, J.A., Bouwmeester, T., Leyns, L., Kertesz, N., Gallo, M., Folletie, M., and De Robertis, E.M. (1997). Cerberus-like is a secreted factor with neuralizing activity expressed in the anterior primitive endoderm of the mouse gastrula. Mech. Dev. (issue cover) 68 :45-57

De Robertis, E.M., Kim, S., Leyns, L., Piccolo, S., Bachiller, D., Agius, E., Belo, J.A. et al. (1997). Patterning by genes expressed in the Spemann’s organizer. Cold Spring Harbor Symp. Quant. Biol. 62 :169-174