Genomics Unit

This Unit has the mission to provide technological support and expertise for research at the genome scale. It composed by the Genotyping and DNA Sequencing services.

The Genotyping Service offers Sequenom iPLEX technology, allowing rapid SNP genotyping assays with up to forty SNPs assayed simultaneously. This MALDI-TOF based platform is suited for genotyping tens to hundreds of SNPs in several thousand DNA samples. Taqman® Technology in a 384-well format is available to users both for SNP genotyping and Real-Time PCR gene expression.

DNA Sequencing Service offers DNA sequencing and fragment analysis using both multicapillary and slab-gel electrophoresis with ABI 3130XL and ABI 377.

Equipment:

Robot PlateMate 2×2 (Matrix) (*)
Five thermocycling machines - ABI 9700 equipped with 2×384 blocks (*)
Chip spotting device (MassARRAY Nanodispenser) (*)
SNP detection, MALDI-TOF technology-MassARRAY Compact (Sequenom)(*)
ABI 3130XL and ABI 377 Automatic Sequencers
7900 HT Fast Real-Time PCR

(*) Financed by Fundação para a Ciência e Tecnologia (Programa Nacional de Re-Equipamento Científico).

For more information visit the GU WebSite